Sirius red is a unique dye that specifically binds to the [Gly-X-Y]n helical structure on fibrillar collagen (type I to V) and does not discriminate between collagen species and types. Chondrex, Inc.
provides a Sirius Red Total Collagen Detection Assay Kit for detecting the total collagen content in various collagen-containing samples such as tissue specimens, cell culture media, and cultured cells. The total assay working time is less than 30 minutes and 40 samples can be measured in duplicate. Due to the low level of collagen in cell culture media, additional concentration steps may be necessary.
For determining levels of collagen from individual species or different types of samples, Chondrex, Inc. recommends our Type I Collagen Detection ELISA kits and Type II Collagen Detection ELISA Kit. For convenience, the same sample preparations may be used for both the Collagen Detection ELISA Kits and the Sirius Red Total Collagen Detection Assay Kit.